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KMID : 0981820100300010034
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Korean Journal of Laboratory Medicine
2010 Volume.30 No. 1 p.34 ~ p.37
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A Report of Cat Scratch Disease in Korea Confirmed by PCR Amplification of the 16S-23S rRNA Intergenic Region of Bartonella henselae
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Suh Bo-Rum
Yang Woo-Ick
Yong Dong-Eun
Kim Dong-Soo
Lee Yang-Soon
Jeong Seok-Hoon
Chun Jin-Kyoung
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Abstract
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We report a case of cat scratch disease in an 8-yr-old girl who presented with fever and enlargement of both axillary lymph nodes. Both aerobic and anaerobic cultures of the lymph node aspirate were negative for microbial growth. Gram staining and Warthin-Starry silver staining did not reveal any organism. Purified DNA from the PCR-amplicon of the 16S-23S rRNA intergenic region was sequenced and showed 99.7% identity with the corresponding sequence of Bartonella henselae strain Houston-1. Our findings suggest that the internal transcribed spacer is a reliable region for PCR identification of Bartonella species. In patients with lymphadenitis, a history of contact with cats or dogs necessitates the use of diagnostic approaches that employ not only the conventional staining and culture but also molecular methods to detect B. henselae.
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KEYWORD
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Bartonella henselae, Cat scratch disease, Polymerase chain reaction
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